-
Product Name
ADRA1B Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ADRA1B
-
Tested applications
WB, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
ADRA1B antibody; ADRA1 antibody; ALPHA1BAR antibody; adrenoceptor alpha 1B antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide of human ADRA1B
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ADRA1B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ADRA1B antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - ADRA1B Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ADRA1B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"