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Product Name
ADRA1A Polyclonal Antibody
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Description
Polyclonal antibody to ADRA1A
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ADRA1A antibody; ADRA1C antibody; ADRA1L1 antibody; ALPHA1AAR antibody; adrenoceptor alpha 1A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 346-475 of human ADRA1A (NP_150646.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - ADRA1A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ADRA1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ADRA1A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using ADRA1A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ADRA1A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using ADRA1A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ADRA1A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using ADRA1A antibody at dilution of 1:100 (40x lens).
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Background
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"