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Product Name
ADAT2 antibody
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Description
ADAT2 Rabbit Polyclonal antibody. Positive IHC detected in human hepat℃irrhosis tissue. Positive IF detected in A431 cells. Positive WB detected in A431 cells, mouse brain tissue, mouse pancreas tissue. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADAT2 antibody; DEADC1 antibody; dJ20N2 antibody; dJ20N2.1 antibody; DKFZp686L1118 antibody; FLJ44213 antibody; TAD2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ADAT2 recombinant protein (Accession Number: BC037955). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:107777(ADAT2 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:107777(ADAT2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:107777(ADAT2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells using Catalog No:107777(ADAT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.
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