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Product Name
ADAMTS5 Polyclonal Antibody
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Description
Polyclonal antibody to ADAMTS5
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
ADAMTS5 antibody; ADAM-TS 11 antibody; ADAM-TS 5 antibody; ADAM-TS5 antibody; ADAMTS-11 antibody; ADAMTS-5 antibody; ADAMTS11 antibody; ADMP-2 antibody; ADAM-TS11 antibody; ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ADAMTS5
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - ADAMTS5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ADAMTS5 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its versican remodeling properties. Participates in development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"