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Product Name
ADAMTS4 Polyclonal Antibody
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Description
Polyclonal antibody to ADAMTS4
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ADAMTS4 antibody; ADAMTS-2 antibody; ADAMTS-4 antibody; ADMP-1 antibody; ADAM metallopeptidase with thrombospondin type 1 motif 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 52-315 of human ADAMTS4 (NP_005090.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ADAMTS4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ADAMTS4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"