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Product Name
ADAM9 Polyclonal Antibody
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Description
Polyclonal antibody to ADAM9
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
ADAM9 antibody; CORD9 antibody; MCMP antibody; MDC9 antibody; Mltng antibody; ADAM metallopeptidase domain 9 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 475-685 of human ADAM9 (NP_003807.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ADAM9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ADAM9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - ADAM9 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ADAM9 antibody . Blue: DAPI for nuclear staining.
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Background
Cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell-matrix interactions and regulate the motility of cells via interactions with integrins.; Isoform 2: May act as alpha-secretase for amyloid precursor protein (APP).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"