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Product Name
ADAM5 antibody
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Description
ADAM5 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue. Positive WB detected in HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 36-50 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ADAM5 antibody; ADAM5P antibody; TMDCII antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ADAM5 recombinant protein (Accession Number: BC067864). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107767(ADAM5P antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:107767(ADAM5P antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:107767(ADAM5P antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:107767(ADAM5P Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ADAM5(putative disintegrin and metalloproteinase domain-containing protein 5) is also named as ADAM5P, TMDC2(tMDCII). The tMDC II, abundantly expressed, sperm surface MDC proteins identified in rodent and macaque systems, is also nonfunctional in the human(PMID:10417343). It has 7 isoforms produced by alternative splicing. It is expressed in testis highly by northern blot. This antibody is specific to ADAM5.
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