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Product Name
ADAM2 Polyclonal Antibody
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Description
Polyclonal antibody to ADAM2
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
ADAM2 antibody; CRYN1 antibody; CRYN2 antibody; CT15 antibody; FTNB antibody; PH-30b antibody; PH30 antibody; PH30-beta antibody; ADAM metallopeptidase domain 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human ADAM2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - ADAM2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ADAM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"