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Product Name
ACTL6A antibody
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Description
ACTL6A Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue. Positive IP detected in HeLa cells. Positive WB detected in K-562 cells, HeLa cells. Observed molecular weight by Western-blot: 45-51kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
actin like 6A antibody; Actin like protein 6A antibody; Actin related protein Baf53a antibody; ACTL6 antibody; ACTL6A antibody; Arp4 antibody; ARPN BETA antibody; ArpNbeta antibody; BAF53 antibody; BAF53A antibody; BRG1 assOCiated factor 53A antibody; INO80 complex subunit K antibody; INO80K antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ACTL6A recombinant protein (Accession Number: NM_004301). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107707(ACTL6A antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary using Catalog No:107707(ACTL6A antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:107707(ACTL6A antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ACTL6A (IP:Catalog No:107707, 4ug; Detection:Catalog No:107707 1:500) with HeLa cells lysate 1200ug.
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Background
Actin-like 6A (ACTL6A, synonyms: ACTL6, BAF53A, MGC5382) is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A is a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix.
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References
- Li S, Liu C, Li N. Genome-wide coactivation analysis of PGC-1alpha identifies BAF60a as a regulator of hepatic lipid metabolism. Cell metabolism. 8(2):105-17. 2008.
- Meng ZX, Li S, Wang L. Baf60c drives glycolytic metabolism in the muscle and improves systemic glucose homeostasis through Deptor-mediated Akt activation. Nature medicine. 19(5):640-5. 2013.
- Meng ZX, Wang L, Chang L. A Diet-Sensitive BAF60a-Mediated Pathway Links Hepatic Bile Acid Metabolism to Cholesterol Absorption and Atherosclerosis. Cell reports. 13(8):1658-69. 2015.
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