Mouse ACVR2B (His & Fc Tag) recombinant protein

Mouse ACVR2B (His & Fc Tag) recombinant protein

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Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor (By similarity).

Activin receptor type-2B

MGC116908; ACTR-IIB; HTX4; ACVR2B; ACTRIIB; MGC118477

P27040

Acvr2b

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Thr 134) of mouse ACVR2B (NP_031423.1) precursor was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse ACVR2B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B/Fc monomer is approximately 60-65 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

1. Measured by its ability to bind biotinylated Human INHBA-his (Cat:10429-H08H) in functional ELISA.
2. Measured by its ability to bind biotinylated mouse INHBA-his (Cat:500709) in functional ELISA.
3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED₅₀ for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.