• Activated Protein C (390-404) (human) peptide

Activated Protein C (390-404) (human) peptide

Not For Human Use, Lab Use Only.

Cat.#: 316067

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Optional Service: TFA RemovalWhat's this?

Special Price 189.8 USD

Availability: 1-2 weeks
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Product Information

  • Product Name
    Activated Protein C (390-404) (human) peptide
  • Documents
  • Sequence Shortening
    YGVYTKVSRYLDWIH
  • Sequence
    H-Tyr-Gly-Val-Tyr-Thr-Lys-Val-Ser-Arg-Tyr-Leu-Asp-Trp-Ile-His-OH
  • Length (aa)
    15
  • Peptide Purity (HPLC)
    96.1%
  • Molecular Formula
    C91H130N22O23
  • Molecular Weight
    1900.17
  • Source
    Synthetic
  • Form
    Powder
  • Description
    Activated protein C (APC), a serine protease, regulates the blood coagulation pathway by inactivating the coagulation cofactors Va and VIIIa. Amino acid residues 390-404 in the APC heavy chain are essential for this anticoagulant activity. The corresponding synthetic peptide as well as anti-peptide antibodies specifically inhibit APC activity in several coagulation assays with half-maximal inhibition at 5 uM peptide and 30 nM antibody, respectively.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
  • References
    • R.M.Mesters et al., J. Biol. Chem., 266, 24514 (1991)
  • About TFA salt

    Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

    TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

    TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

    In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Peptide Property

  • Analysed Sequence:H-YGVYTKVSRYLDWIH-OH
  • Chemical Formula:C91H130N22O23
  • Sequence length:15
  • Extinction coefficient:9530 M-1cm-1
  • GRAVY:-0.34
  • Mw average:1900.13
  • Theoretical pI:9.39
  • Data Source:Peptide Property Calculator

GRAVY = grand average of hydropathy

X: Hydrophobic uncharged residues, like F I L M V W A and P

X: Basic residues, like R K H

X: Acidic residues, like D E

X: Polar uncharged residues, like G S T C N Q and Y

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"