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Product Name
ACSS2 Polyclonal Antibody
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Description
Polyclonal antibody to ACSS2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat, Monkey
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Alternative names
ACSS2 antibody; ACAS2 antibody; ACECS antibody; ACS antibody; ACSA antibody; dJ1161H23.1 antibody; acetyl-coenzyme A synthetase, cytoplasmic antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human ACSS2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ACSS2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ACSS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - ACSS2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using ACSS2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACSS2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using ACSS2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ACSS2 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using ACSS2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Activates acetate so that it can be used for lipid synthesis or for energy generation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"