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Product Name
ACPP antibody
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Description
ACPP Mouse Monoclonal antibody. Positive WB detected in Human testis. Positive IHC detected in human prostate hyperplasia tissue. Observed molecular weight by Western-blot: 45-50kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
acid phosphatase antibody; prostate antibody; ACP 3 antibody; ACP3 antibody; ACPP antibody; PAP antibody; Prostatic acid phosphatase antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of ACPP recombinant protein (Accession Number: NM_001134194). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:107558(ACPP antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human prostate hyperplasia using Catalog No:107558(ACPP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate hyperplasia using Catalog No:107558(ACPP antibody) at dilution of 1:50 (under 40x lens)
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Background
ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"