-
Product Name
ACOT8 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ACOT8
-
Tested applications
WB
-
Species reactivity
Human
-
Alternative names
ACOT8 antibody; HNAACTE antibody; NAP1 antibody; PTE-1 antibody; PTE-2 antibody; PTE1 antibody; PTE2 antibody; hACTE-III antibody; hTE antibody; acyl-CoA thioesterase 8 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACOT8 (NP_005460.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - ACOT8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ACOT8 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
-
Background
Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20). Inactive towards substrates with more than C20 aliphatic chains. Involved in the metabolic regulation of peroxisome proliferation.; (Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"