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Product Name
ACOT2 antibody
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Description
ACOT2 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skeletal muscle tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in human brain tissue, HEK-293 cells, HepG2 cells, human kidney tissue, human testis tissue, mouse kidney tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 46-53 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACOT2 antibody; acyl CoA thioesterase 2 antibody; CTE Ia antibody; Mte1 antibody; PTE2 antibody; PTE2A antibody; ZAP128 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ACOT2 recombinant protein (Accession Number: NM_006821). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107745(ACOT2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:107745(ACOT2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:107745(ACOT2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ACOT2 (IP:Catalog No:107745, 3ug; Detection:Catalog No:107745 1:500) with HepG2 cells lysate 1700ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:107745(ACOT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ACOT2 represents acyl-CoA thioesterase 2
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