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Product Name
ACOT12 antibody
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Description
ACOT12 Rabbit Polyclonal antibody. Positive IF detected in COLO 320 cells. Positive IHC detected in human liver cancer tissue, human colon cancer tissue. Positive WB detected in COLO 320 cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACOT12 antibody; acyl CoA thioesterase 12 antibody; Cach antibody; CACH 1 antibody; CACH1 antibody; hCACH 1 antibody; STARD15 antibody; THEAL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ACOT12 recombinant protein (Accession Number: NM_130767). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107744(ACOT12 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107744(ACOT12 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107744(ACOT12 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed COLO 320 cells using Catalog No:107744(ACOT12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ACOT12 represents acyl-CoA thioesterase 12
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"