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Product Name
Acetyl-Histone H2A-K9 mAb
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Description
Monoclonal antibody to Acetyl-Histone H2A-K9
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A specific peptide of human Acetyl-Histone H2A-K9
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:500 - 1:1000
IF 1:100 - 1:500 -
Validations
Western blot - Acetyl-Histone H2A-K9 mAb
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K9 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - Acetyl-Histone H2A-K9 mAb
Immunohistochemistry of paraffin-embedded mouse colon using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Acetyl-Histone H2A-K9 mAb
Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Acetyl-Histone H2A-K9 mAb
Immunohistochemistry of paraffin-embedded human tonsil using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Acetyl-Histone H2A-K9 mAb
Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Acetyl-Histone H2A-K9 mAb
Immunohistochemistry of paraffin-embedded human lung using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Acetyl-Histone H2A-K9 mAb
Immunohistochemistry of paraffin-embedded mouse thyroid using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Acetyl-Histone H2A-K9 mAb
Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2A-K9 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Acetyl-Histone H2A-K9 mAb
Immunofluorescence analysis of MCF-7 cells using Acetyl-Histone H2A-K9 antibody .
Immunofluorescence - Acetyl-Histone H2A-K9 mAb
Immunofluorescence analysis of A549 cells using Acetyl-Histone H2A-K9 antibody .
Immunofluorescence - Acetyl-Histone H2A-K9 mAb
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K9 antibody .
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Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"