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Product Name
ACAT1 Polyclonal Antibody
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Description
Polyclonal antibody to ACAT1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ACAT1 antibody; ACAT antibody; MAT antibody; T2 antibody; THIL antibody; acetyl-CoA acetyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ACAT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ACAT1 antibody at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - ACAT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using ACAT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACAT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using ACAT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACAT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using ACAT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACAT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat spleen using ACAT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACAT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using ACAT1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ACAT1 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using ACAT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ACAT1 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ACAT1 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Plays a major role in ketone body metabolism.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"