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Product Name
ACADS Polyclonal Antibody
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Description
Polyclonal antibody to ACADS
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
ACADS antibody; ACAD3 antibody; SCAD antibody; acyl-CoA dehydrogenase, C-2 to C-3 short chain antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - ACADS Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ACADS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunofluorescence - ACADS Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using ACADS antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - ACADS Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ACADS antibody . Western blot was performed from the immunoprecipitate using ACADS antibody at a dilition of 1:1000.
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Background
Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i.e. less than 6 carbon atoms.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"