ACADS Polyclonal Antibody

Cat.#: 165261

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Product Information

  • Product Name
    ACADS Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ACADS
  • Tested applications
    WB, IF, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ACADS antibody; ACAD3 antibody; SCAD antibody; acyl-CoA dehydrogenase, C-2 to C-3 short chain antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
    IP 1:50 - 1:100
  • Validations

    Western blot - ACADS Polyclonal Antibody

    Western blot - ACADS Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ACADS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.

    Immunofluorescence - ACADS Polyclonal Antibody

    Immunofluorescence - ACADS Polyclonal Antibody

    Immunofluorescence analysis of MCF7 cells using ACADS antibody . Blue: DAPI for nuclear staining.

    Immunoprecipitation - ACADS Polyclonal Antibody

    Immunoprecipitation - ACADS Polyclonal Antibody

    Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ACADS antibody . Western blot was performed from the immunoprecipitate using ACADS antibody at a dilition of 1:1000.

  • Background
    Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i.e. less than 6 carbon atoms.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"