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Product Name
ACADS antibody
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Description
ACADS Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human heart tissue. Positive IP detected in HEK-293 cells. Positive WB detected in human brain tissue, COS-7 cells, HEK-293 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
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Alternative names
ACAD3 antibody; ACADS antibody; Butyryl CoA dehydrogenase antibody; SCAD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ACADS recombinant protein (Accession Number: NM_000017). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107721(ACADS antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:107721(ACADS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:107721(ACADS antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ACADS (IP:Catalog No:107721, 4ug; Detection:Catalog No:107721 1:500) with HEK-293 cells lysate 3000ug.
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Background
ACADS is also named as SCAD(Short-chain specific acyl-CoA dehydrogenase, mitochondrial) and belongs to the acyl-CoA dehydrogenase family. It is a tetrameric mitochondrial flavoprotein. It is synthesized as a 44 kDa precursor, transported into mitochondria, and proteolytically processed to its 41 kDa mature form. Acyl-CoA de- hydrogenases (ACADs) are a family of flavoproteins that are involved in theβ-oxidation of the fatty acyl-CoA derivatives.
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References
- Xu T, Zheng L, Xu L. Protective effects of dioscin against alcohol-induced liver injury. Archives of toxicology. 88(3):739-53. 2014.
- Huang D, Li T, Li X. HIF-1-mediated suppression of acyl-CoA dehydrogenases and fatty acid oxidation is critical for cancer progression. Cell reports. 8(6):1930-42. 2014.
- Dong D, Qi Y, Xu L. Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats. Food & function. 5(12):3065-75. 2014.
- Liu M, Xu L, Yin L. Potent effects of dioscin against obesity in mice. Scientific reports. 5:7973. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"