ACADM Polyclonal Antibody

Cat.#: 163610

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Product Information

  • Product Name
    ACADM Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ACADM
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ACADM antibody; ACAD1 antibody; MCAD antibody; MCADH antibody; acyl-CoA dehydrogenase, C-4 to C-12 straight chain antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACADM (NP_000007.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - ACADM Polyclonal Antibody

    Western blot - ACADM Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ACADM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

    Immunofluorescence - ACADM Polyclonal Antibody

    Immunofluorescence - ACADM Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using ACADM antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

  • Background
    Acyl-CoA dehydrogenase specific for acyl chain lengths of 4 to 16 that catalyzes the initial step of fatty acid beta-oxidation. Utilizes the electron transfer flavoprotein (ETF) as an electron acceptor to transfer electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"