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Product Name
ACAD10 antibody
- Documents
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Description
ACAD10 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue. Positive IF detected in Hela cells. Positive WB detected in mouse spleen tissue. Observed molecular weight by Western-blot: 119kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACAD 10 antibody; ACAD10 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ACAD10 recombinant protein (Accession Number: BC015056). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:107825(ACAD10 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:107825(ACAD10 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:107825(ACAD10 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ACAD10 antibody Catalog No:107825 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ACAD10(Acyl-CoA dehydrogenase family member 10) belongs to the acyl-CoA dehydrogenase family. ACAD10 has significant activity towards the branched-chain substrates R and S, 2 methyl-C15-CoA and is highly expressed in fetal but not adult brain. This pattern of expression is similar to that of LCAD, another ACAD previously shown to be involved in long branched chain fatty acid metabolism(PMID:21237683). It has 4 isoforms produced by alternative splicing.
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