Ac-SDKP peptide

Ac-SDKP peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-SDKP-OH

Ac-Ser-Asp-Lys-Pro-OH

4

95.07%

C₂₀H₃₃N₅O₉

487.5

Synthetic

Powder

Ac-SDKP is a natural tetrapeptide with anti-inflammatory and antifibrotic properties. It has been widely distributed in mammalian organs, plasma, urine, and circulating mononuclear cells. The peptide is hydrolyzed by angiotensin-converting enzyme (ACE) and has a short half-life of 4.5 min in the circulation. Treatment with ACE inhibitors significantly increases plasma concentration of Ac-SDKP, and some of the ACE inhibitors protective effects were reported to be mediated by the increases in Ac-SDKP concentrations. In animal diseases with hypertension, as well as cardiovascular and kidney disease, Ac-SDKP reduces inflammatory cell infiltration and collagen deposition in the heart, kidney, lung, and liver. The peptide has potential applications in the treatment of various inflammatory and fibrotic diseases.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Kumar N, Nakagawa P, Janic B, Romero CA, Worou ME, Monu SR, Peterson EL, Shaw J, Valeriote F, Ongeri EM, Niyitegeka JM, Rhaleb NE, Carretero OA. The anti-inflammatory peptide Ac-SDKP is released from thymosin-β4 by renal meprin-α and prolyl oligopeptidase. Am J Physiol Renal Physiol. 2016 May 15;310(10):F1026-34. doi: 10.1152/ajprenal.00562.2015. Epub 2016 Mar 9. PMID: 26962108; PMCID: PMC4889319.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.