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Product Name
ABL2 antibody
- Documents
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Description
ABL2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells. Positive IHC detected in human liver cancer tissue, human gliomas tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 128 kDa-140 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Abelson related gene protein antibody; ABL2 antibody; ABLL antibody; ARG antibody; FLJ22224 antibody; FLJ31718 antibody; FLJ41441 antibody; Tyrosine protein kinase ABL2 antibody; Tyrosine protein kinase ARG antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ABL2 recombinant protein (Accession Number: NM_005158). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107818(ABL2 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using ABL2 antibody Catalog No:107818 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-ABL2 (IP:Catalog No:107818, 4ug; Detection:Catalog No:107818 1:500) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107818(ABL2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:107818(ABL2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"