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Product Name
ABCG8 Polyclonal Antibody
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Description
Polyclonal antibody to ABCG8
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ABCG8 antibody; GBD4 antibody; STSL antibody; ATP binding cassette subfamily G member 8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ABCG8 (NP_071882.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ABCG8 Polyclonal Antibody
Western blot analysis of extracts of A-549 cells, using ABCG8 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - ABCG8 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ABCG8 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"