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Product Name
ABCE1 Polyclonal Antibody
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Description
Polyclonal antibody to ABCE1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ABCE1 antibody; ABC38 antibody; OABP antibody; RLI antibody; RLI1 antibody; RNASEL1 antibody; RNASELI antibody; RNS4I antibody; ATP binding cassette subfamily E member 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-599 of human ABCE1 (NP_002931.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ABCE1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ABCE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - ABCE1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ABCE1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"