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Product Name
ABCB11 Polyclonal Antibody
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Description
Polyclonal antibody to ABCB11
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ABCB11 antibody; ABC16 antibody; BRIC2 antibody; BSEP antibody; PFIC-2 antibody; PFIC2 antibody; PGY4 antibody; SPGP antibody; bile salt export pump antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human ABCB11 (NP_003733.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ABCB11 Polyclonal Antibody
Western blot analysis of extracts of rat liver, using ABCB11 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 1s.
Immunofluorescence - ABCB11 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using ABCB11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ABCB11 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using ABCB11 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"