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Product Name
ABCA4 Polyclonal Antibody
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Description
Polyclonal antibody to ABCA4
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ABCA4 antibody; ABC10 antibody; ABCR antibody; ARMD2 antibody; CORD3 antibody; FFM antibody; RMP antibody; RP19 antibody; STGD antibody; STGD1 antibody; ATP binding cassette subfamily A member 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 110-300 of human ABCA4 (NP_000341.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ABCA4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ABCA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - ABCA4 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ABCA4 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"