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Product Name
AATF antibody
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Description
AATF Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 63kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AATF antibody; CHE 1 antibody; CHE1 antibody; DED antibody; Protein AATF antibody; Rb binding protein Che 1 antibody; Traube antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AATF recombinant protein (Accession Number: NM_012138). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107678(AATF antibody) at dilution of 1:800
Immunofluorescent analysis of Hela cells, using AATF antibody Catalog No:107678 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"