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Product Name
AARS2 Polyclonal Antibody
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Description
Polyclonal antibody to AARS2
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
AARS2 antibody; AARSL antibody; COXPD8 antibody; LKENP antibody; MT-ALARS antibody; MTALARS antibody; alanine--tRNA ligase, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 841-985 of human AARS2 (NP_065796.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - AARS2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AARS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.
Immunohistochemistry - AARS2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using AARS2 antibody at dilution of 1:100 (40x lens).
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Background
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"