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Product Name
AARE antibody
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Description
AARE Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive WB detected in mouse liver tissue, COLO 320 cells, HeLa cells, Jurkat cells. Observed molecular weight by Western-blot: 66-80kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AARE antibody; ACPH antibody; Acyl peptide hydrolase antibody; Acylaminoacyl peptidase antibody; APEH antibody; APH antibody; D3F15S2 antibody; D3S48E antibody; DNF15S2 antibody; OPH antibody; Oxidized protein hydrolase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AARE recombinant protein (Accession Number: NM_001640). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:107674(APEH antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107674(APEH antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107674(APEH antibody) at dilution of 1:100 (under 40x lens)
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Background
APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH.
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