AADAT Polyclonal Antibody

Cat.#: 161434

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Product Information

  • Product Name
    AADAT Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to AADAT
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    AADAT antibody; KAT2 antibody; KATII antibody; KYAT2 antibody; aminoadipate aminotransferase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-429 of human AADAT (NP_001273611.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:1000 - 1:2000
  • Validations

    Western blot - AADAT Polyclonal Antibody

    Western blot - AADAT Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using AADAT antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"