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Product Name
AADAC Polyclonal Antibody
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Description
Polyclonal antibody to AADAC
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
AADAC antibody; CES5A1 antibody; DAC antibody; arylacetamide deacetylase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-399 of human AADAC (NP_001077.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - AADAC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using AADAC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.
Immunofluorescence - AADAC Polyclonal Antibody
Immunofluorescence analysis of 293T cells using AADAC antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
Displays cellular triglyceride lipase activity in liver, increases the levels of intracellular fatty acids derived from the hydrolysis of newly formed triglyceride stores and plays a role in very low-density lipoprotein assembly. Displays serine esterase activity in liver. Deacetylates a variety of arylacetamide substrates, including xenobiotic compounds and procarcinogens, converting them to the primary arylamide compounds and increasing their toxicity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"