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6*his,His tag antibody

Cat.#: 107663

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Special Price 239.0 USD

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Product Information

  • Product Name
    6*his,His tag antibody
  • Documents
  • Description
    6*his,His tag Rabbit Polyclonal antibody. Positive WB detected in Recombinant protein.
  • Tested applications
    ELISA, WB
  • Species reactivity
    Tag
  • Alternative names
    6 his antibody; 6*his antibody; His tag antibody; his tag antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Recombinant Protein. Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:5000

  • Validations

    Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (Catalog No:117296) at various dilutions.

    Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (Catalog No:117296) at various dilutions.

  • Background
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
  • References
    • Fei X, Gu X, Fan S. Crystal structure of Human ASB9-2 and substrate-recognition of CKB. The protein journal. 31(4):275-84. 2012.
    • Béaslas O, Vihervaara T, Li J, Laurila PP, Yan D, Olkkonen VM. Silencing of OSBP-related protein 8 (ORP8) modifies the macrophage transcriptome, nucleoporin p62 distribution, and migration capacity. Experimental cell research. 318(15):1933-45. 2012.
    • Chen S, Chen K, Zhang Q, Cheng H, Zhou R. Regulation of the transcriptional activation of the androgen receptor by the UXT-binding protein VHL. The Biochemical journal. 456(1):55-66. 2013.
    • Gui X, Li R, Zhang X. An important amino acid in nucleoprotein contributes to influenza A virus replication by interacting with polymerase PB2. Virology. 464-465:11-20. 2014.
    • Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 20:43-51. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"