Rat CD73 / NT5E (His Tag) recombinant protein

Rat CD73 / NT5E (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

5' nucleotidase, ecto

CALJA; AI447961; NT5; ECTO-5'-NUCLEOTIDASE; E5NT; NT; 2210401F01RIK; NTE; ENT; RP11-321N4.1; CD73; 5'-NT; EN; 5'-NUCLEOTIDASE; NT5E

Q66HL0

Nt5e; rCG_25342

Human Cells

A DNA sequence encoding the rat NT5E(Q66HL0) (Met1-Lys549) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat NT5E comprises 532 amino acids and predicts a molecular mass of 59.4 kDa. The apparent molecular mass of the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >15,000 pmol/min/µg.