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Product Name
NT5E/CD73 antibody
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Description
NT5E/CD73 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A375 cells, human spleen tissue, K-562 cells, mouse brain tissue, rat brain tissue. Positive FC detected in NCCIT cell. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, WB, FC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
5 NT antibody; 5 nucleotidase antibody; 5' nucleotidase antibody; ecto (CD73) antibody; CD73 antibody; E5NT antibody; Ecto 5 nucleotidase antibody; eNT antibody; NT5 antibody; NT5E antibody; NTE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NT5E/CD73 recombinant protein (Accession Number: BC015940). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113396(NT5E,CD73 antibody) at dilution of 1:400
1X10^6 NCCIT cell were stained with 0.2ug NT5E,CD73 antibody (Catalog No:113396, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).
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