3IH3 peptide

3IH3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-IHHIHHIIHHIHHIIHHIHHI-NH2

H-Ile-His-His-Ile-His-His-Ile-Ile-His-His-Ile-His-His-Ile-Ile-His-His-Ile-His-His-Ile-NH2

21

95.2%

C₁₂₆H₁₈₆N₄₆O₂₁

2681.11

Synthetic

Powder

The pH-dependent antimicrobial activity of α-helical coiled coil peptides is caused by the protonation/deprotonation of histidine residues in peptide molecules under different pH environments and then leads to the gain or loss of the binding ability of LPS and membranes. 3IH3 has the activity against Gram-negative bacteria. 3IH3 was also found to be effective against C. albicans 2.2086, C. tropicalis 2.1975, and C. parapsilosis 2.3989 with registering MIC values of 2, 1, and 2 μM at pH 6.0, respectively. 3IH3 had no cytotoxicity against HEK293T cells. At the concentration of 128 μM, 3IH3 induced more than a statistically 99% cell survival rate at both pH values. 3IH3 shows great stability as an inexpensive and effective antimicrobial activity agent and has the potential for clinical application in the treatment of infections occurring in body sites with acidic pH.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lai Z, Tan P, Zhu Y, Shao C, Shan A, Li L. Highly Stabilized u03b1-Helical Coiled Coils Kill Gram-Negative Bacteria by Multicomplementary Mechanisms under Acidic Condition. ACS Appl Mater Interfaces. 2019;11(25):22113-22128

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.