14-3-3 Mouse Monoclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in rat brain tissue. Positive WB detected in human brain tissue, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 31 kDa,28 kDa
ELISA, WB, IHC, IF, FC
Human, Rat; other species not tested.
14 3 3 antibody; 14 3 3 protein T cell antibody; 14 3 3 protein tau antibody; 14 3 3 protein theta antibody; 14-3-3 tau antibody; HS1 antibody; Protein HS1 antibody; YWHAQ antibody
This antibody was obtained by immunization of 14-3-3 recombinant protein (Accession Number: NM_006826). Purification method: Protein G purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107550(14-3-3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded rat brain tissue slide using Catalog No:107550(14-3-3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded rat brain tissue slide using Catalog No:107550(14-3-3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107550(14-3-3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (Catalog No:107550, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.
14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. 66061-1-Ig recognizes 14-3-3 tau (YWHAQ), eta (YWHAH), and zeta (YWHAZ), but not epsilon (YWHAE).
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