(IRIP)7-NH2 peptide

(IRIP)7-NH2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

IRPIIRPIIRPIIRPIIRPIIRPIIRPI-NH2 (amidation)

H-Ile-Arg-Pro-Ile-Ile-Arg-Pro-Ile-Ile-Arg-Pro-Ile-Ile-Arg-Pro-Ile-Ile-Arg-Pro-Ile-Ile-Arg-Pro-Ile-Ile-Arg-Pro-Ile-NH2

28

95.2%

C₁₆₁H₂₉₀N₅₀O₂₈

3374.33

Synthetic

Powder

A set of novel peptides was synthesized based on an antitrypsin/antichymotrypsin hydrolytic peptide structure unit (XYPX) n (X represents I, L, and V; Y represents R and K), which was designed using a systematic natural amino acid arrangement. Of these peptides, 16 with seven repeat units had the highest average selectivity index (GMSI = 99.07) for all of the Gram-negative bacteria tested and remained highly effective in combating Escherichia coli infection in vivo. Importantly, 16 also had dramatic resistance to a high concentration of trypsin/chymotrypsin hydrolysis and exerted bactericidal activity through a membrane-disruptive mechanism. Overall, these findings provide new approaches for the development of antiprotease hydrolytic peptides that target Gram-negative bacteria.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Wang J, Song J, Yang Z, He S, Yang Y, Feng X, Dou X, Shan A. Antimicrobial Peptides with High Proteolytic Resistance for Combating Gram-Negative Bacteria. J Med Chem. 2019 Mar 14;62(5):2286-2304.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.