Protease Digestion Tool

1. Enter sequence (Input the raw sequence, not fasta format):

Full length:0

2. Select a protease/chemical:

Digest your protein substrates with customizable specificity

This tool models the hydrolitic behaviour of a proteolytic enzyme. Its main purpose is to predict the outcome of hydrolitic cleavage of a peptidic substrate.

The enzyme specificity is currently modeled for 39 enzymes/reagents. This models are somewhat simplistic as they are largely regex-based, and do not take into account subtleties such as kinetic/temperature effects, accessible solvent area, secondary or tertiary structure elements.

For a complete description of their specificities, you can check out Peptide Cutter.

Currently implemented specificities:

  • Alcalase
  • Arg-C proteinase
  • Asp-N endopeptidase
  • Asp-N endopeptidase + N-terminal Glu
  • BNPS-Skatole
  • Caspase1
  • Caspase2
  • Caspase3
  • Caspase4
  • Caspase5
  • Caspase6
  • Caspase7
  • Caspase8
  • Caspase9
  • Caspase10
  • Chymotrypsin-high specificity (C-term to [FYW], not before P)
  • Chymotrypsin-low specificity (C-term to [FYWML], not before P)
  • Clostripain (Clostridiopeptidase B)
  • CNBr
  • Enterokinase
  • Factor Xa
  • Formic acid
  • Glutamyl endopeptidase
  • GranzymeB
  • Hydroxylamine
  • HCl
  • Iodosobenzoic acid
  • LysC
  • LysN
  • NTCB (2-nitro-5-thiocyanobenzoic acid)
  • Pepsin (pH1.3)
  • Pepsin (pH>2)
  • Proline-endopeptidase
  • Proteinase K
  • Staphylococcal peptidase I
  • Thermolysin
  • Thrombin
  • Tobacco etch virus protease
  • Trypsin