Digest your protein substrates with customizable specificity
This tool models the hydrolitic behaviour of a proteolytic enzyme. Its main purpose is to predict the outcome of hydrolitic cleavage of a peptidic substrate.
The enzyme specificity is currently modeled for 39 enzymes/reagents. This models are somewhat simplistic as they are largely regex-based, and do not take into account subtleties such as kinetic/temperature effects, accessible solvent area, secondary or tertiary structure elements.
For a complete description of their specificities, you can check out Peptide Cutter.
Currently implemented specificities:
- Alcalase
- Arg-C proteinase
- Asp-N endopeptidase
- Asp-N endopeptidase + N-terminal Glu
- BNPS-Skatole
- Caspase1
- Caspase2
- Caspase3
- Caspase4
- Caspase5
- Caspase6
- Caspase7
- Caspase8
- Caspase9
- Caspase10
- Chymotrypsin-high specificity (C-term to [FYW], not before P)
- Chymotrypsin-low specificity (C-term to [FYWML], not before P)
- Clostripain (Clostridiopeptidase B)
- CNBr
- Enterokinase
- Factor Xa
- Formic acid
- Glutamyl endopeptidase
- GranzymeB
- Hydroxylamine
- HCl
- Iodosobenzoic acid
- LysC
- LysN
- NTCB (2-nitro-5-thiocyanobenzoic acid)
- Pepsin (pH1.3)
- Pepsin (pH>2)
- Proline-endopeptidase
- Proteinase K
- Staphylococcal peptidase I
- Thermolysin
- Thrombin
- Tobacco etch virus protease
- Trypsin