Peptide Library Design Tool

0. Select the type of the peptide library:

1. Enter sequence (Input the raw sequence, not fasta format):

Full length:0

Peptide Library

Overlapping Peptide Library – This tool allows you to generate overlapping peptide fragments to cover the full length of your protein of interest. You are able to define sequence parameters such as peptide length and number of amino acids offset. The designed sequences can be edited and exported to either word or Excel format.

Alanine Scanning Library – This tool allows you to systematically substitute each amino acid position on your peptide sequences with alanine- the smallest of the naturally occurring amino acids that maintains it chirality. Alanine mutagenesis can be a powerful tool for probing the effect of individual amino acids side chain to protein function and properties.

Truncation Library – This tool allows you to build your peptide library by systematically removing flanking amino acids to determine the minimum length required for optimum activity. Once the essential amino acid residues for protein activity are identified by alanine scanning, the direction of truncation can be selected around these residues, rather than removing residues from both ends of the peptide sequence.

Positional Scanning Library – This tool allows you to optimize the peptide lead sequence. A selected position on the lead peptide is replaced with different amino acid (natural or unnatural) to improve the peptide properties such as biological activity, enzyme stability and solubility. The substitution can be performed on a single or multiple positions on a peptide sequence. A peptide library constructed using positional scanning can be very informative in studying protein interfaces whether in epitope mapping or protein-protein interaction.

Random Library - This tool allows you to create a library which is similar to positional scanning library. It’s constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity

Scramble Library - Scramble Library is constructed by carrying out permutation on the original peptide’s sequence. It has the potential to give all possible alternatives and offers and represents the highest degree of variability for peptide library

T-Cell Library - T-cell truncated peptide libraries allow the testing of all possible T-cell epitopes across a protein of interest.