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Calculate dissociation constant from ΔG

Calculate dissociation constant(Kd) from ΔG

1. Input ΔG:

2. Input temperature: K



Binding constant

Consider reaction:R + L ⇌ RL, we haveKon[R][L]=Koff[R][L]

then equilibrium association constant Ka = Kon/Koff,the reciprocal of Ka is equilibrium dissociation constant(Kd)。Binding affinity is typically measured and reported by the equilibrium dissociation constant。

Kd has a quantitative relationship with ΔG(molar Gibbs free energy):ΔG=RTlnKd

the relation between ΔG and Kd at 298K(25℃)

ΔG(kcal/mol) Kd(mol/L)
-12 1.56E-09
-11.5 3.64E-09
-11 8.47E-09
-10.5 1.97E-08
-10 4.59E-08
-9.5 1.07E-07
-9 2.49E-07
-8.5 5.79E-07
-8 1.35E-06
-7.5 3.14E-06
-7 7.30E-06
-6.5 1.70E-05
-6 3.95E-05
-5.5 9.20E-05
-5 2.14E-04
-4.5 4.99E-04
-4 1.16E-03