To prepare L Zeocin Stock Solution
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Zeocin | 100 g | 100 mg/mL | C55H85O21N20S2Cu·HCl/1526.5 | 11006-33-0 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 100 g of Zeocin to the solution.
- Add distilled water until the volume is 1 L.
- Prewet a 0.22 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. Sterilize Zeocin Stock through the prepared 0.22 µm syringe filter. Stock may be kept at -20°C for 1 year. Bacterial cells (E. Coli, etc): 25-50 µg/mL; Mamamlian Cells (cell line dependent): 50-400 µg/mL; For yeast, 100 µg/mL is sufficient.