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Tris/EACA buffer Preparation and Recipe

To prepare L  Tris/EACA buffer

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Tris base1.211 g0.01 MC4H11NO3/121.1477-86-1
ε-aminocaproic acid (EACA)11.802 g0.089 MC6H13NO2/131.1760-32-2
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 1.211 g of Tris base to the solution.
  3. Add 11.802 g of ε-aminocaproic acid (EACA) to the solution.
  4. Add distilled water until the volume is 1 L.