Tris-buffered saline (TBS) is an excellent wash buffer for many types of immunoassays.
To prepare L Tris-Buffered Saline (TBS) 10×
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Tris base | 24.228 g | 0.2 M | C4H11NO3/121.14 | 77-86-1 |
Sodium chloride | 87.66 g | 1.5 M | NaCl/58.44 | 7647-14-5 |
- Prepare 0.8 L of H2O in a suitable container.
- Add 24.228 g of Tris base to the solution.
- Add 87.66 g of Sodium chloride to the solution.
- Add H2O until the volume is 1 L.
- Adjust pH to 7.6 with 12 N HCl before adjusting to final volume with water. Prepared TBS is stable at 4 °C for 3 months.