SSC (saline sodium citrate) buffer is commonly used in Northern blotting as a hybridization or wash buffer.
To prepare L SSC for Northerns (20×)
Table 1. Recipe
| Name | Amount | Concentration | Formula/M.W. | CAS # |
|---|---|---|---|---|
| Sodium chloride | 175.32 g | 3 M | NaCl/58.44 | 7647-14-5 |
| 77.42 g | 0.3 M | C6H5O7Na3/258.07 | 68-04-2 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 175.32 g of Sodium chloride to the solution.
- Add 77.42 g of Sodium citrate to the solution.
- Adjust the pH to 7.0.
- Add distilled water until the volume is 1 L.
- Autoclave the solution and store it at room temperature. It will last for 1 year or longer.