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Sodium Phosphate Buffer Preparation and Recipe

The buffer is one of the most popular currently used, and is commonly employed in molecular and cell biology, chemistry, and material science, among many others.

To prepare L  Sodium Phosphate Buffer

M

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
2.231 g0.018 MNaH2PO4/119.987558-80-7
11.555 g0.081 MNa2HPO4/141.967558-79-4
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 2.231 g of Sodium dihydrogen phosphate to the solution.
  3. Add 11.555 g of Sodium phosphate dibasic to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Phosphate buffer is highly water soluble and has a high buffering capacity, but will inhibit enzymatic activity and precipitates in ethanol.