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Sodium Carbonate Transfer Buffer (40×, pH 9.5) Preparation and Recipe

To prepare L  Sodium Carbonate Transfer Buffer (40×, pH 9.5)

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Sodium bicarbonate21.086 g0.251 MNaHCO3/84.01144-55-8
18.336 g0.173 MNa2CO3/105.99497-19-8
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 21.086 g of Sodium bicarbonate to the solution.
  3. Add 18.336 g of Sodium carbonate to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Store at room temperature.