To prepare L SDS-PAGE SDS Running Buffer (10x)
Table 1. Recipe
Name | Amount | Concentration | Formula/M.W. | CAS # |
---|---|---|---|---|
Tris base | 30.285 g | 0.25 M | C4H11NO3/121.14 | 77-86-1 |
Glycine | 144.4 g | 1.923 M | NH2CH2COOH/75.07 | 56-40-6 |
Sodium dodecyl sulfate (SDS) | 10 g | 1% w/v | C12H25O4NaS/288.38 | 151-21-3 |
- Prepare 0.8 L of distilled water in a suitable container.
- Add 30.285 g of Tris base to the solution.
- Add 144.4 g of Glycine to the solution.
- Add 10 g of Sodium dodecyl sulfate (SDS) to the solution.
- Add distilled water until the volume is 1 L.