Potassium Phosphate Buffer Preparation and Recipe

Potassium phosphate buffers, sometimes called Gomori buffers, consist of a mixture of monobasic dihydrogen phosphate and dibasic monohydrogen phosphate.

To prepare L  Potassium Phosphate Buffer

Table 1. Recipe

1. Prepare 0.8 L of distilled water in a suitable container.
2. Add 2.531 g of Potassium dihydrogen phosphate to the solution.
3. Add 14.178 g of Potassium phosphate dibasic to the solution.
4. Add distilled water until the volume is 1 L.
5. Phosphate buffer is highly water soluble and has a high buffering capacity, but will inhibit enzymatic activity and precipitates in ethanol.